Toni's Protocols

Gene and Operon Synthesis Analysis

Bacterial Promoter Calculator (Softberry)
Finding Terminators (ARNold)
mRNA folding (RNAfold)
Bacterial Genome Integration (Recombineering)

Biotransformation

Biotransformation

Protein Expression

Small Prep with Sigma His Select Column
24 Well Plate Protein Expression
Large Scale Protein Purification
Yeast Microsome Isolation
Yeast Display Expression
Yeast Secretion Expression
Ammonium Sulfate Precipitation
TEV Cleavage
DynaBeads

Cloning

Bacterial Transformations
Colony PCR
Fast Digest DpnI
Electrocompetent Cell Transformation
General Hot Start Phusion Kit PCR
Gibson Cloning
PIPE Cloning
Qiagen Buffers
Q-trays
Quikchange Mutagenesis
Quikchange Without the Kit
Quikchange Lightning Multisite Kit
Quikchange Multisite Without the Kit
Round the Horn Mutagenesis
Round the Horn for Library Creation
Competent Cells
Subclone into pPNL6
Traditional Cloning
Yeast Transformation
Yeast Miniprep
Zymo Mini Prep

Libraries
Mini Gene Assembly Library
Replica Plating

Gel Electrophoresis

DNA Loading Dye
SDS Loading Dye Recipe

Assays
Circular Dichroism
qPCR Thermostability Assay
Avicel Park Johnson Activity Screen
Congo Red Cellulase Activity Screen
Park and Johnson Assay
T50 Assay
60 Hour Assay
Terpene Synthase Assay
Hemochrome Assay

Crystallography
Installing Coot
Omit Map and General Maps

Useful Info
Finnzymes Tm Calculator
Protein Properties
Phosphate Buffer Calculator
Hot Start Phusion Manual
Ni-NTA Agarose Bead Regeneration
Toni's Products

Yeast
Cellulase Yeast Plates and Media
Yeast Display Expression
Dropout Media
FACS
Minimal Media
Yeast Frozen Competent Cells
Yeast Genomic DNA Isolation
Yeast Rich Media
Yeast Miniprep

Computational
Foldx
Gromacs
AutoDock
Getting and Updating Triad
Non-Contiguous Recombination
Triad Core Design
Statistical Coupling Analysis

CD
CD

Robot
Using the Robot

EPA Protocols

ABI Prism 310 Sequencer
DNA Isolation Protocol with Qiagen Stool Kit
DNA Isolation Protocol with Trizol